논문 구분 * |
학술지명* | 논문명* | ISSN* | DOI | 기여율(%) | 주저자명 (제1저자)* |
공동저자명 | 볼륨 번호* |
SCI(E) 구분* |
논문페이지 | 학술지 임팩트팩터 |
학술지 출판일자 |
초록 | |
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시작* | 끝 | |||||||||||||
국외학술지 | Annals of the Entomological Society of America | First reports of Vespa mandarinia (Hymenoptera: Vespidae) in North America represent two separate maternal lineages in Washington State, United States, and British Columbia, Canada | 0013-8746 | 10.1093/aesa/saaa024 | Telissa M Wilson | Junichi Takahashi, Sven-Erik Spichiger, Iksoo Kim, Paul van Westendorp | 113 | SCI(E) | 468 | 472 | 1.510 | 20200907 | In September 2019, destruction of a Vespa mandarinia Smith 1852 nest was reported for the first time in North America in Nanaimo, British Columbia, Canada. In December 2019, the Washington State Department of Agriculture also confirmed the first detection of an adult specimen of V. mandarinia in the United States, in Whatcom County, Washington. Vespa mandarinia is the largest hornet species and is a known predator of several insects, including the European honey bee (Apis mellifera) (Hymenoptera: Apidae) (Linnaeus, 1758). The establishment of V. mandarinia in North America poses a serious threat to apiculture, and this species is considered an actionable quarantine pest. Here we report details of the first detection of this species in the United States and use genetic sequence data obtained from five specimens across the globe to estimate differences in origin of the Canadian and U.S. detections. The full mitochondrial genomes of four V. mandarinia specimens representing different geographic locations were sequenced and compared with an existing reference genome. A maximum likelihood tree using 13 protein-coding regions from mitochondrial DNA suggests that the Canada and U.S. specimens are from two separate maternal lineages. A large-scale survey is currently underway to assess the level of Asian giant hornet establishment in both countries and to determine the future direction of eradication efforts. | |
국외학술지 | INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES | Functional characterization of a putative RNA demethylase ALKBH6 in Arabidopsis growth and abiotic stress responses | 1661-6596 | 10.3390/ijms21186707 | 83.3 | Trinh Thi Huong | Le Nguyen Tieu Ngoc | 21(18) | SCI(E) | 6701(1) | 6701(14) | 4.556 | 20200913 | RNA methylation and demethylation, which is mediated by RNA methyltransferases (referred to as “writers”) and demethylases (referred to as “erasers”), respectively, are emerging as a key regulatory process in plant development and stress responses. Although several studies have shown that AlkB homolog (ALKBH) proteins are potential RNA demethylases, the function of most ALKBHs is yet to be determined. The Arabidopsis thaliana genome contains thirteen genes encoding ALKBH proteins, the functions of which are largely unknown. In this study, we characterized the function of a potential eraser protein, ALKBH6 (At4g20350), during seed germination and seedling growth in Arabidopsis under abiotic stresses. The seeds of T-DNA insertion alkbh6 knockdown mutants germinated faster than the wild-type seeds under cold, salt, or abscisic acid (ABA) treatment conditions but not under dehydration stress conditions. Although no di erences in seedling and root growth were observed between the alkbh6 mutant and wild-type under normal conditions, the alkbh6 mutant showed a much lower survival rate than the wild-type under salt, drought, or heat stress. Cotyledon greening of the alkbh6 mutants was much higher than that of the wild-type upon ABA application. Moreover, the transcript levels of ABA signaling-related genes, including ABI3 and ABI4, were down-regulated in the alkbh6 mutant compared to wild-type plants. Importantly, the ALKBH6 protein had an ability to bind to both m6A-labeled and m5C-labeled RNAs. Collectively, these results indicate that the potential eraser ALKBH6 plays important roles in seed germination, seedling growth, and survival of Arabidopsis under abiotic stresses. |
국외학술지 | International Journal of Molecular Sciences | IKKγ/NEMO Is Required to Confer Antimicrobial Innate Immune Responses in the Yellow Mealworm, Tenebrio Molitor | 1422-0067 | 10.3390/ijms21186734 | 100 | Hye Jin Ko | Yong Hun Jo; Maryam Keshavarz; Bharat Bhusan Patnaik; Ki Beom Park; Chang Eun Kim; Ho Am Jang; Yong Seok Lee; Yeon Soo Han | 21(18) | SCI(E) | 6734 | 6734 | 4.566 | 20200914 | IKKγ/NEMO is the regulatory subunit of the IκB kinase (IKK) complex, which regulates the NF-κB signaling pathway. Within the IKK complex, IKKγ/NEMO is the non-catalytic subunit, whereas IKKα and IKKβ are the structurally related catalytic subunits. In this study, TmIKKγ was screened from the Tenebrio molitor RNA-Seq database and functionally characterized using RNAi screening for its role in regulating T. molitor antimicrobial peptide (AMP) genes after microbial challenges. The TmIKKγ transcript is 1521 bp that putatively encodes a polypeptide of 506 amino acid residues. TmIKKγ contains a NF-κB essential modulator (NEMO) and a leucine zipper domain of coiled coil region 2 (LZCC2). A phylogenetic analysis confirmed its homology to the red flour beetle, Tribolium castaneum IKKγ (TcIKKγ). The expression of TmIKKγ mRNA showed that it might function in diverse tissues of the insect, with a higher expression in the hemocytes and the fat body of the late-instar larvae. TmIKKγ mRNA expression was induced by Escherichia coli, Staphylococcus aureus, and Candida albicans challenges in the whole larvae and in tissues such as the hemocytes, gut and fat body. The knockdown of TmIKKγ mRNA significantly reduced the survival of the larvae after microbial challenges. Furthermore, we investigated the tissue-specific induction patterns of fourteen T. molitor AMP genes in TmIKKγ mRNA-silenced individuals after microbial challenges. In general, the mRNA expression of TmTenecin1, -2, and -4; TmDefensin1 and -2; TmColeoptericin1 and 2; and TmAttacin1a, 1b, and 2 were found to be downregulated in the hemocytes, gut, and fat body tissues in the TmIKKγ-silenced individuals after microbial challenges. Under similar conditions, TmRelish (NF-κB transcription factor) mRNA was also found to be downregulated. Thus, TmIKKγ is an important factor in the antimicrobial innate immune response of T. molitor. |
국외학술지 | Journal of Microbiology and Biotechnology | Anti-Inflammatory Activity of Antimicrobial Peptide Periplanetasin-5 Derived from the Cockroach Periplaneta Americana | 1017-7825 | 10.4014/jmb.2004.04046 | In-Woo Kim | Joon Ha Lee, Minchul Seo, Hwa Jeong Lee, Minhee Baek, Mi-Ae Kim, Yong Pyo Shin, Sung Hyun Kim, Iksoo Kim, Jae Sam Hwang | 30 | SCI(E) | 1282 | 1289 | 1.992 | 20200928 | Previously, we performed an in silico analysis of the Periplaneta americana transcriptome. Antimicrobial peptide candidates were selected using an in silico antimicrobial peptide prediction method. It was found that periplanetasin-5 had antimicrobial activity against yeast and grampositive and gram-negative bacteria. In the present study, we demonstrated the anti-inflammatory activities of periplanetasin-5 in mouse macrophage Raw264.7 cells. No cytotoxicity was observed at 60 μg/ml periplanetasin-5, and treatment decreased nitric oxide production in Raw264.7 cells exposed to lipopolysaccharide (LPS). In addition, quantitative RT-PCR and enzyme-linked immunosorbent assay revealed that periplanetasin-5 reduced cytokine (tumor necrosis factor-α, interleukin-6) expression levels in the Raw264.7 cells. Periplanetasin-5 controlled inflammation by inhibiting phosphorylation of MAPKs, an inflammatory signaling element, and reducing the degradation of IκB. Through LAL assay, LPS toxicity was found to decrease in a periplanetasin-5 dose-dependent manner. Collectively, these data showed that periplanetasin-5 had antiinflammatory activities, exemplified in LPS-exposed Raw264.7 cells. Thus, we have provided a potentially useful antibacterial peptide candidate with anti-inflammatory activities. | |
국외학술지 | Entomological Research | Bacterial but not fungal challenge up-regulates the transcription of Coleoptericin genes in Tenebrio molitor | 1738-2297 | 10.1111/1748-5967.12465 | 100 | Ho Am Jang | Ki Beom Park; Bo Bae Kim; Maryam Ali Mohammadie Kojour; Young Min Bae; Snigdha Baliarsingh; Yong Seok Lee; Yeon Soo Han; Yong Hun Jo | 50(9) | SCI(E) | 440 | 449 | 0.807 | 20200929 | Abstract Antimicrobial peptides (AMPs) are effector candidates that elicit humoral immunity in invertebrates. AMPs facilitate bacterial clearance by either physically disrupting the microbial membranes or the intracellular targets. In the Coleopteran pest, Tenebrio molitor, transcriptional regulation of AMPs has been studied in the context of innate immune signaling cascades and antimicrobial immunity. Here, we report the transcriptional response of three AMP genes, Coleoptericin A, B, and C (TmCole A, B and C) in T. molitor in response to bacterial (Escherichia coli, Staphylococcus aureus), and fungal (Candida albicans) challenges. These genes were expressed essentially in the gut and hemocytes followed by the integument tissue of the T. molitor larva. Further, these genes were highly expressed in the late-larval, pupal, and early adult stages. Furthermore, while all of these transcripts were highly upregulated in the fat body and Malpighian tubules after bacterial challenge, TmCole B and TmCole C were induced in the gut after E. coli challenge. Fungal stimulation was not required for the upregulation of the transcription of Coleoptericin genes in T. molitor. |
국외학술지 | Systematic Entomology | How well do multispecies coalescent methods perform with mitochondrial genomic data? A case study of butterflies and moths (Insecta: Lepidoptera) | 0307-6970 | 10.1111/syen.12431 | Min Jee Kim | Iksoo Kim, Stephen L. Cameron | 45 | SCI(E) | 857 | 873 | 3.909 | 20201001 | Despite the broad adoption of multispecies coalescent (MSC) methods for nuclear phylogenomics, they have yet to be applied to mitochondrial (mt) genomic data. As the potential sources of phylogenomic bias that MSC methods can address, such as incomplete lineage sorting, horizontal gene transfer and gene tree heterogeneity, have been found in mt genomic data, these approaches may improve the accuracy of phylogenetic inference with these data. In the present study, we examined the behaviour of MSC methods in reconstructing the phylogeny of Lepidoptera (butterflies and moths), a group for which mt genomic data are known to have strong resolving power. Traditional concatenation methods of analysing mt genomes for Lepidoptera infer topologies highly congruent with those generated from independent nuclear datasets. Individual mt gene trees performed poorly in recovering consensus relationships at deep levels (i.e. superfamily monophyly and inter‐relationships) and only moderately well for shallow relationships (i.e. within Papilionoidea). In contrast, MSC analyses with ASTRAL performed strongly with almost complete concordance to both concatenated mt genome analyses and independent nuclear analyses at both deep and shallow phylogenetic scales. Outgroup choice had a limited impact on tree accuracy, with even phylogenetically distant outgroups still resulting in topologies highly congruent with results from nuclear datasets, although MSC analyses appeared to be marginally more affected by outgroup choice than concatenation analyses. In general, discordance between concatenation and MSC analyses was found at nodes whose resolution varied between previous nuclear phylogenomic studies. The sensitivity of individual relationships to analysis with MSC vs concatenation can thus be used to test the robustness of phylogenetic hypotheses. For insect phylogenetics, MSC is a reliable inference method for mt genomic data and is thus a useful complement to the already widely used concatenation approaches. | |
국내학술지 | JOURNAL OF PLANT BIOLOGY | A La-related protein LaRP6a delays flowering of Arabidopsis thaliana by upregulating FLC transcript levels | 1226-9239 | 10.1007/s12374-020-09261-7 | 75 | Su Jung Park | Hwa Jung Lee, Kwanuk Lee | 63(5) | SCI(E) | 369 | 378 | 1.529 | 20201001 | La-related proteins (LaRPs) are RNA-binding proteins (RBPs) that contain a La-type helix-turn-helix (HTH) domain and are found in all eukaryotes, including plants, animals, and yeasts. Although the functions of a few LaRPs have been determined during plant growth and development, the roles of most LaRPs in plants remain unknown. Here, the function of an Arabidopsis thaliana LaRP6a (At5g46250) harboring a La-type HTH domain and an RNA-recognition motif was determined in the growth and development of Arabidopsis. Confocal analysis of subcellular localization of the LaRP6a?GFP fusion protein revealed that LaRP6a protein is localized to both the nucleus and plasma membrane. Overexpression of LaRP6a resulted in delayed flowering and increased plant height and seed yield. The transcript level of the floral repressor FLC was markedly increased in LaRP6a-overexpressing transgenic plants. LaRP6a protein bound to the 3?UTR of the FLC transcript and possessed RNA chaperone activity. Collectively, these results suggest that LaRP6a plays a role in the control of flowering time by regulating FLC transcript levels. |
국외학술지 | Molecular and cellular probes | Rapid detection of peach latent mosaic viroid by reverse transcription recombinase polymerase amplification | 0890-8508 | 10.1016/j.mcp.2020.101627 | 100 | hyo-Jeong Lee | Hyun-Ju Kim, Keumhee Lee, Rae-Dong Jeong | 53 | SCI(E) | 101627 | 101627 | 1.951 | 20201030 | Reverse transcription recombinase polymerase amplification (RT-RPA), an isothermal nucleic acid amplification and detection method, was developed to detect peach latent mosaic viroid (PLMVd) in pollen and peach leaves. Results showed that this RT-RPA detection method can be performed at 42℃ and completed in approximately 5 min, and there was no cross-reactivity with other common peach viruses. A sensitivity assay showed that the RTRPA assay was 1000-fold more sensitive than a regular RT-PCR. Moreover, the method was successfully applied to test field-collected samples. The newly developed RT-RPA assay is rapid, sensitive, and reliable method for detection of PLMVd in peach pollen and leaves and can be utilized as an effective technique in quarantine and viroid-free certification processes. |
국외학술지 | The Plant Pathology Journal | Reverse Transcription Recombinase Polymerase Amplification Assay for Rapid and Sensitive Detection of Barley Yellow Dwarf Virus in Oat | 1598-2254 | 10.5423/PPJ.NT.08.2020.0148. | 100 | Na-Kyeong Kim | Sang-Min Kim, Rae-Dong Jeong | 36(5) | SCI(E) | 497 | 502 | 1.570 | 20201030 | Barley yellow dwarf virus (BYDV) is an economically important plant pathogen that causes stunted growth, delayed heading, leaf yellowing, and purple leaf tip, thereby reducing the yields of cereal crops worldwide. In the present study, a reverse transcription recombinase polymerase amplification (RT-RPA) assay was developed for the detection of BYDV in oat leaf samples. The RT-RPA assay involved incubation at an isothermal temperature (42°C) and could be performed rapidly in 5 min. In addition, no cross-reactivity was observed to occur with other cereal-infecting viruses, and the method was 100 times more sensitive than conventional reverse transcription polymerase chain reaction. Furthermore, the assay was validated for the detection of BYDV in both field-collected oat leaves and viruliferous aphids. Thus, the RT-RPA assay developed in the present study represents a simple, rapid, sensitive, and reliable method for detecting BYDV in oats. |
국외학술지 | The Plant Pathology Journal | Detection of Plant Pathogenic Viruses in Commercial Gochujang (Fermented Red Pepper Paste) from Korea | 1598-2254 | 10.5423/PPJ.NT.06.2020.0093 | 100 | Seo-Yeon Go | Na-Kyeong Kim, Hyo-Jeong Lee, Tae-Ho Ryu, Jin-Sung Hong, Rae-Dong Jeong | 36(5) | SCI(E) | 503 | 508 | 1.570 | 20201030 | The potential transmission of plant pathogenic viruses through processed foods could be a source of concern for global crop production; however, there is a lack of supporting evidence. The present study was conducted to investigate the presence of plant athogenic viruses in five samples of gochujang (fermented red pepper paste) manufactured in Korea. Several viruses infecting pepper were detected by reverse transcriptionpolymerase chain reaction, among which the pepper mild mottle virus (PMMoV) was detected in all five samples, at concentrations ranging from 2.8 to 7.0 (log10 copies/ml). In addition, PMMoV was observed by transmission electron microscopy in all five samples. The samples exhibited viral pathogenicity to Nicotiana benthamiana plants, indicating that global trade of processed products could be a possible source of the transmission of plant viruses. |
국외학술지 | ACTA PHYSIOLOGIAE PLANTARUM | Identification, characterisation and expression analysis of MADS-box genes in sweetpotato wild relative Ipomoea trifida | 1861-1664 | 10.1007/s11738-020-03153-6 | 75 | Panpan Zhu | Tingting Dong, Tao Xu | 42(11) | SCI(E) | 163(1) | 163(13) | 1.76 | 20201101 | MADS-box transcription factors (TFs) participate in various biological processes, including stress response, plant growth and development. The storage root of sweetpotato (Ipomoea batatas) is an important staple food crop in Asian and African areas, and leafy sweetpotato is a popular and healthy vegetable in China. However, the function of the MADS genes remains largely unknown in sweetpotato and the other Ipomoea species. In this study, we carried out a genome-wide gene family analysis of MADS in Ipomoea trifida which is currently considered as the most potential ancestor of sweetpotato. A total of 37 ItfMADS genes that encode 42 proteins were identified. The evolutionary relationship was analysed for MADS proteins in 37 species. According to the tree topology of phylogeny, the ItfMADS genes are classified into Mα, Mβ, Mγ, MIKCC and MIKC* groups. Gene structure analysis revealed that the majority of ItfMADS in the same clade displayed similar exon?intron distribution. Furthermore, the expression patterns of ItfMADS genes were investigated through RNA-seq data and confirmed by qRT-PCR. The expression of ItfMADS genes varied among different tissues, and induced or repressed under different abiotic stresses, thereby suggesting their potential functions under abiotic stresses. Our findings lay a solid foundation for further elucidating the ItfMADS function and promoting sweetpotato molecular breeding. |
국외학술지 | Mitochondrial DNA Part B-Resources | Complete mitochondrial genome of Pterodecta felderi (Lepidoptera: Callidulidae) | 2380-2359 | 10.1080/23802359.2020.1833777 | Keon Hee Lee | Min Jee Kim, Jeong Sun Park, Iksoo Kim | 5 | SCI(E) | 3730 | 3732 | 0.885 | 20201111 | We report the mitochondrial genome (mitogenome) of Pterodecta felderi (Callidulidae: Lepidoptera), which is the first mitogenome sequences in the family Callidulidae, a monotypic family in the superfamily Calliduloidea. The 15,340-bp long complete mitogenome consists of a typical set of genes (13 protein-coding genes [PCGs], 2 rRNA genes, and 22 tRNA genes) and 1 major non-coding A + T-rich region, which are arranged in a way that is frequently observed in Lepidoptera. Of the 13 PCGs, 12 P. felderi start with ATN, except for COI, which starts with CGA. The P. felderi mitogenome consists of 210-bp long intergenic-spacer sequences and 27-bp long overlaps. Phylogenetic analysis of superfamilial relationships in the lepidopteran clade Obtectomera with concatenated sequences of the 13 PCGs and 2 rRNA genes using the Bayesian inference method showed that Calliduloidea, which is only represented by P. felderi, was placed as the most basal lineage about Macroheterocera (Lasiocampoidea, Bombycoidea, Mimallonoidea, Noctuoidea, and Drepanoidea), Papilionoidea, and Pyraloidea. | |
국외학술지 | Mitochondrial DNA Part B-Resources | Mitochondrial genome of mason bee, Osmia pedicornisa (Hymenopetra: Megachilidae) | 2380-2359 | 10.1080/23802359.2020.1833775 | Hyung Joo Yoon | Jeong Sun Park, Su Yeon Jeong, Kyeong Yong Lee, Iksoo Kim | 5 | SCI(E) | 3764 | 3766 | 0.885 | 20201111 | The mason bee, Osmia pedicornis Cockerell, 1919, which is importantly used as the pollinator, particularly for apples in Korea. We sequenced the mitochondrial genome (mitogenome) of O. pedicornis as an initial study for species identification and subsequent population genetic study. The size of the incomplete genome was 14,505 bp, excluding the trnA, trnC, and the A + T-rich region that were unable to sequence, but including partially sequenced trnM and srRNA. The genome included typical sets of protein-coding genes (PCGs), rRNA genes, and one non-coding region, tRNAs, excluding two unidentified tRNAs. Although positions of the two tRNAs that were not sequenced are unknown the gene arrangement of O. pedicornis mitogenome has the tRNA arrangement, trnM-trnQ-trnI, at the A + T-rich region and ND2 junction that differed from that of previously published O. excavate, which has trnA-trnQ-trnI arrangement at the junction. Phylogenetic analyses were performed using concatenated sequences of the 13 PCGs genes and the maximum likelihood method with the inclusion of a total of 12 mitogenome sequences belonging to three families in the superfamily Apoidea. Current O. pedicornis was placed as the sister to the O. bicornis, with the highest nodal support. The Apidae and Megachilidae were placed as the sister group, with the placement of Colletidae as the basal lineage for the group with the highest nodal support. | |
국외학술지 | Mitochondrial DNA Part B-Resources | The complete mitochondrial genome of Ricania speculum (Walker, 1851) (Hemiptera: Ricaniidae): investigation of intraspecific variations on mitochondrial genome | 2380-2359 | 10.1080/23802359.2020.1839366 | Hyobin Lee | Jonghyun Park, Hong Xi, Gwan-Seok Lee, Iksoo Kim, Jongsun Park, Wonhoon Lee | 5 | SCI(E) | 3814 | 3816 | 0.885 | 20201120 | We have determined a mitochondrial genome of Ricania speculum (Walker, 1851) collected in Jeollabuk-do, Republic of Korea. The circular mitogenome of R. speculum is 15,530 bp long which is shorter than that of the previous mitogenome of R. speculum by 199 bp. It includes 13 protein-coding genes, two ribosomal RNA genes, and 22 transfer RNAs. Intraspecific variation between two mitogenome of R. speculum was investigated: 171 SNPs and 18 INDELs were identified, presenting a high level of intraspecific variations on mitochondrial genome. | |
국외학술지 | Journal of Plant Pathology | First report of cucumber mosaic virus nfecting Aster scaber in Korea | 1125-4653 | 10.1007/s42161-020-00651-x | 100 | Seo-Yeon Go | Na-Kyeong Kim, Ji-Soo Park, Jin-Sung Hong, Rae-Dong Jeong | 102 | SCI(E) | 1373 | 1374 | 1.152 | 20201201 | Aster scaber (family Asteraceae), a perennial herb that grows wild in the dry mountain regions of Korea, eastern Russia, China, and Japan, can potentially have many applications because of its anticancer, anti-inflammatory, and antioxidant properties. In September 2019, virus-like symptoms, including mosaic, chlorosis and malformation of leaves, were observed on A. scaber plants at a farm located in Gwangju, Korea. To identify the causal agent, serological assays with commercially available double-antibody sandwich (DAS)-ELISA kits specific for cucumber mosaic virus (CMV), cucumber green mottle mosaic virus, pepper mottle virus, pepper mild mottle virus, tomato mosaic virus, and watermelon mosaic virus (Agdia, Elkhart, IN, USA) were conducted with symptomatic leaves collected randomly from 40 different plants. Only CMV was detected, in 50% of the tested samples. To confirm the presence of CMV, RT-PCR was performed with specific primers CPTALL-5′ (5′-YASYTTTDRGGTTCAATTCC-3′) and CPTALL-3′ (5′-GACTGACCATTTTAGCCG-3′) that amplified a 937 bp product containing the complete coat protein (CP) gene (Choi et al., 1999). |
국외학술지 | Entomological Research | In silico identification and expression analyses of Defensin genes in the mealworm beetle Tenebrio molitor | 1738-2297 | 10.1111/1748-5967.12468 | 100 | Ho Am Jang | Ki Beom Park; Bo Bae Kim; Maryam Ali Mohammadie Kojour; Young Min Bae; Snigdha Baliarsingh; Yong Seok Lee; Yeon Soo Han; Yong Hun Jo | 50(12) | SCI(E) | 575 | 585 | 0.807 | 20201229 | Abstract Defensins are a major family of antimicrobial peptides that serve as the innate immune defense of both vertebrates and invertebrates. Due to their antimicrobial, chemotactic, and regulatory activities, Defensins have been exploited for their therapeutic potential. Insect Defensins are cysteine-rich and contain an N-terminal loop, α-helix, and antiparallel β-sheet, forming a “cysteine-stabilized alpha beta (CSαβ)” or “loop–helix-sheet” structure. In this study, we identified the full-length open reading frame (ORF) sequences of Defensin (TmDef) and Defensin-like (TmDef-like) genes from the mealworm beetle Tenebrio molitor using in silico methods. TmDef and TmDef-like genes encode the peptides of 72 and 71 amino acid residues, respectively. TmDefensin is comprised of a Defensin domain and the TmDefensin-like is comprised of a signal peptide of 21 amino acid residues. Phylogenetic analysis revealed close similarities of TmDefensin with the Defensin of Acalolepta luxuriosa of the longhorn beetle family. The expression of TmDef mRNA was found to be greater than that of TmDef-like mRNA and was mostly expressed in the pupal and adult stages. Tissue distribution showed high expression of TmDef-like mRNA in larval hemocytes, gut, integument, and fat body, while in adults, the expression was high in gut and hemocytes. Following bacterial and fungal stimulation in vivo, TmDef was upregulated at 24 h post-infection in whole body, fat body, and hemocytes of the larvae. Even TmDef-like mRNA was upregulated in the gut and hemocytes at 12 and 9 h post-infection respectively. These results suggest that TmDef and TmDef-like genes play important roles in protecting T. molitor from microbial contact. |
국외학술지 | Horticultural Science and Technology |
Reverse Transcription Loop-mediated Isothermal Amplification Assay for Detecting Peach Latent Mosaic Viroid in Peach Pollen | 1226-8763 | 10.7235/HORT.20200075 | 100 | hyo-Jeong Lee | Rae-Dong Jeong | 38(6) | SCI(E) | 830 | 839 | 0.695 | 20201231 | Artificial pollination is used to increase the yield of many fruit crops. However, much of the pollen used for this process is imported from other countries, thereby increasing the risk of introducing plant pathogens, such as peach latent mosaic viroid (PLMVd). According to the Animal and Plant Quarantine Service in Korea, PLMVd is a quarantine pathogen that reduces both fruit yield and tree vigor. Therefore, the aim of the present study was to develop a reverse transcription-loop-mediated isothermal amplification (RT-LAMP) assay for detecting PLMVd in imported pollen. The optimum RT-LAMP conditions were incubation at 63°C for 60 min, and the assay was both highly specific and 10 times more sensitive than RT-PCR. The usefulness of the RT-LAMP assay was also confirmed by screening both imported pollen and leaf samples for PLMVd. Thus, the present study demonstrates the usefulness of RT-LAMP for the rapid screening of PLMVd in field conditions, peach certification programs, and quarantine inspections. |